User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/01: Difference between revisions
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*Calibration curves did not turn out as expected (linear trend) | *Calibration curves did not turn out as expected (linear trend) | ||
*DNA, not MB, should be in excess for DNA-MB samples to ensure all MB is bound | *DNA, not MB, should be in excess for DNA-MB samples to ensure all MB is bound | ||
*Focus on three concentrations, run each sample several times, average curves | *Focus on three concentrations, run each sample several times, average curves based on best spectra taken today | ||
**Oligo D: 150, 125, 100pM run three times at 500s per sample | **Oligo D: 150, 125, 100pM run three times at 500s per sample | ||
**DNA/MB: 100pM DNA/80pM MB, 75/60, 50/40pM run three times at 500s per sample (increase to 700s per sample if spectra are noisey) | **DNA/MB: 100pM DNA/80pM MB, 75/60, 50/40pM run three times at 500s per sample (increase to 700s per sample if spectra are noisey) |
Revision as of 13:14, 1 July 2013
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July 1, 2013FCS Sample Preparation
FCS Data: Calibration CurvesNotes
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