User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/22: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 20: Line 20:
*[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/1/celldetachmentprotocol.pdf Company procedure]
*[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/1/celldetachmentprotocol.pdf Company procedure]


===Test Results===
====Type I Collagen Test====
====Trypsin Cell Detachment Procedure & Cell Counting====
*“Original” - 125 cells on scaffold
*Polydopmaine & “original” - 450 cells on scaffold
*Polydopmaine only- 150 cells on scaffold
*Brute force - 300 cells on scaffold
*Plain - 100 cells on scaffold
===Next time===
*Perform all tests in triplicate




Revision as of 10:06, 22 April 2014

Cell Tests <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

April 22, 2014

Objectives

  • Cell tests

Test Procedures

Type I Collagen Test (Detection and Quantification)

Collagen Staining Procedure & Microscope Imaging

Trypsin Cell Detachment Procedure & Cell Counting

Test Results

Type I Collagen Test

Trypsin Cell Detachment Procedure & Cell Counting

  • “Original” - 125 cells on scaffold
  • Polydopmaine & “original” - 450 cells on scaffold
  • Polydopmaine only- 150 cells on scaffold
  • Brute force - 300 cells on scaffold
  • Plain - 100 cells on scaffold

Next time

  • Perform all tests in triplicate



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/04/22&oldid=789751"