User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/09/26

Nanofiber Coated Scaffolds

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September 26, 2014 Procedures Solution Prep Au stock solution: 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM Myoglobin stock solution: 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM Nanofiber Synthesis Protein Ratio Final [Au] Vol Au Vol Protein Vol Water Myoglobin 100:1 0.5 mM 0.895 mL 0.403 mL 3.702 mL 0.25 mM 0.448 mL 0.201 mL 4.351 mL Measure solutions into 10 mL glass test tubes 8 solutions total Stack 4 of the same type of scaffold into each tube Heat at 80C for 4 hours