User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Tests</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Tests</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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===Test Procedures=== | ===Test Procedures=== | ||
====Type I Collagen Test (Detection and Quantification)===== | =====Type I Collagen Test (Detection and Quantification)===== | ||
*[http://www.chondrex.com/products/mouse-type-i-collagen-detection-kit Company procedure ] | *[http://www.chondrex.com/products/mouse-type-i-collagen-detection-kit Company procedure ] | ||
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=====Collagen Staining Procedure & Microscope Imaging Test===== | =====Collagen Staining Procedure & Microscope Imaging Test===== | ||
*Absorbance data: | *Absorbance data: | ||
[[Image:2014_0422_abs_collagen_quantification_test.PNG]] | |||
*Calculations (from company procedure) | *Calculations (from company procedure) | ||
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**Collagen (ug/3 scaffold layers)= [OD 540 value-(OD 605 value x 0.291)]/0.0378 | **Collagen (ug/3 scaffold layers)= [OD 540 value-(OD 605 value x 0.291)]/0.0378 | ||
**Non-collagenous proteins (ug/3 scaffold layers)= OD 605 value/0.00204 | **Non-collagenous proteins (ug/3 scaffold layers)= OD 605 value/0.00204 | ||
[[Image:2014_0422_collagen_quantification_calcs.png]] | |||
=====Trypsin Cell Detachment Procedure & Cell Counting===== | =====Trypsin Cell Detachment Procedure & Cell Counting===== | ||
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===Next time=== | ===Next time=== | ||
*Perform all three tests in triplicate | *Perform all three tests in triplicate | ||
*Have one scaffold for assays that has no cells one it (for blank & with "leftover" media [under microscope?]) | |||
*Keep solutions post-assay to look at under microscope to see if cells are still there | |||
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Latest revision as of 23:54, 26 September 2017
Cell Tests | Main project page Previous entry Next entry |
April 22, 2014Objectives
Test ProceduresType I Collagen Test (Detection and Quantification)Collagen Staining Procedure & Microscope ImagingTrypsin Cell Detachment Procedure & Cell CountingTest ResultsCollagen Staining Procedure & Microscope Imaging Test
Trypsin Cell Detachment Procedure & Cell CountingPerformed on 3 scaffold layers.
Next time
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