User:Klare Lazor/Notebook/Chem-496-001/2013/04/12: Difference between revisions
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==Objective== | ==Objective== | ||
* Read plates | * Read plates | ||
* took florescence of the differ concentrations used with hydrogel pieces and determined the concentrations of dye attached | * took florescence of the differ concentrations used with hydrogel pieces and determined the concentrations/mols of dye attached | ||
==Description== | ==Description== | ||
'''NOTE: Mass of whole hydrogel: 12.1419 grams, piece of hydrogel 0.8 grams, so instead of 0.193 grams to occupy all the butayne-1-ol sites(0.000017mols) an amount of 0.014 grams of dye is needed.''' | |||
*the stock solution of dye is 5mg/ml=5g/L therefore the amount of grams of dye in vial, as well as assuming 1:1 reaction between butane-1-ol and dye then a successful reaction will have a percent reacted ~ equal to the percentages below. Percentages below are equal to the amount of dye in mols added to the film/the amount of mols that should react with the butane-1-ol times 100. | |||
**'''1µL of Dye'''= 0.000005g= 8.9E-9 mol/0.000017mols= 0.0523% | |||
**'''10µL of Dye'''= 0.00005g=8.9E-8 mols/0.000017= 0.523% | |||
**'''100µL of Dye'''= 0.0005g=8.9E-7mols/.000017= 5.23% | |||
**'''200µl of dye'''= 0.001g= 1.8E-6mols/.000017= 10.5% | |||
**'''500µl of dye'''= .0025g= 4.4E-6 mols/.000017= 25.8% | |||
'''1µL of Dye''' | '''1µL of Dye''' | ||
number of mols of dye | number of mols of dye | ||
*starting solution: 8.9E- | *starting solution: 8.9E-9 mols | ||
*after soaking for 24hours with gel: 1.34E-9 mols | *after soaking for 24hours with gel: 1.34E-9 mols | ||
*leached in water solution after 24 hours: 4.3919E-9 mols | *leached in water solution after 24 hours: 4.3919E-9 mols | ||
**8.9E- | **8.9E-9mols- 1.34E-9mols=7.56E-9mols of dye soaked up by gel in 24 hours | ||
** | ** 7.56E-9mols - 4.3919E-9mols= '''3.17E-9 mols of dye in hydrogel actual''' | ||
** | ** mols butyne-1-ol sites in small piece of hydrogel (assuming 1:1 reaction): 0.000017 | ||
*Percent of dye reacted with butyne-1-ol=0.0186% | |||
'''10µL of Dye''' | '''10µL of Dye''' | ||
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*leached in water solution after 24 hours: 3.86E-9 | *leached in water solution after 24 hours: 3.86E-9 | ||
**8.9E-8 mols- 8.8E-9mols= 8.03E-8 mols of dye soaked up by gel in 24 hours | **8.9E-8 mols- 8.8E-9mols= 8.03E-8 mols of dye soaked up by gel in 24 hours | ||
** 8.03E-8mols - 3.86E-9mols= '''7.64E-8 of dye | ** 8.03E-8mols - 3.86E-9mols= '''7.64E-8 mols of dye in hydrogel actual''' | ||
** | ** mols of butyne-1-ol sites in small piece of hydrogel: 0.000017 | ||
*Percent of dye reacted with butyne-1-ol= 0.45% '''error possibility or slice of film had more sites''' | |||
'''100µL of Dye''' | |||
number of mols of dye | |||
*starting solution: 8.9E-7mols | |||
*after soaking for 24hours with gel: 1.608E-7mols | |||
*leached in water solution after 24 hours: 3.856E-9 mols | |||
**8.9E-7 mols- 1.608E-7mols= 7.3E-7 mols of dye soaked up by gel in 24 hours | |||
** 7.3E-7mols - 3.86E-7mols= '''7.26E-7 mols of dye in hydrogel actual''' | |||
**mols of butyne-1-ol sites in small piece of hydrogel: 0.000017 | |||
*Percent of dye reacted with butyne-1-ol= 4.27% '''Error possibility or slice of film had more sites''' | |||
'''200µl of dye''' | |||
number of mols of dye | |||
*starting solution: 1.8E-6mols | |||
*after soaking for 24hours with gel: 4.6E-7mols | |||
*leached in water solution after 24 hours: 1.6E-7 mols | |||
**1.8E-6 mols- 4.6E-7mols= 1.34E-6 mols of dye soaked up by gel in 24 hours | |||
** 1.34E-6mols - 1.6E-7mols= '''1.18E-6 mols of dye in hydrogel actual''' | |||
**mols of butyne-1-ol sites in small piece of hydrogel: 0.000017 | |||
*Percent of dye reacted with butyne-1-ol= 6.94% | |||
'''500µl of dye''' | |||
number of mols of dye | |||
*starting solution: 4.4E-6mols | |||
*after soaking for 24hours with gel: 8.8E-7 mols | |||
*leached in water solution after 24 hours: 8.4E-7 mols | |||
**4.4E-6 mols- 8.8E-7mols= 3.5E-6 mols of dye soaked up by gel in 24 hours | |||
** 3.5E-6mols - 8.4E-7mols= '''2.67E-6 mols of dye in hydrogel actual''' | |||
** mols of butyne-1-ol sites in small piece of hydrogel: 0.000017 | |||
*Percent of dye reacted with butyne-1-ol= 15.71% | |||
==Data== | ==Data== |
Revision as of 17:55, 17 April 2013
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Objective
DescriptionNOTE: Mass of whole hydrogel: 12.1419 grams, piece of hydrogel 0.8 grams, so instead of 0.193 grams to occupy all the butayne-1-ol sites(0.000017mols) an amount of 0.014 grams of dye is needed.
Data
NotesThis area is for any observations or conclusions that you would like to note.
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