User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/23

From OpenWetWare
Jump to navigationJump to search
AU CHEM-570 Lab Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Bench work

  1. Analytical minigel
    1. 10μL 1KB ladder
    2. --
    3. Hartings PCR sample
    4. --
    5. --
    6. 10μL gel-purified, double-digested pTXB1 from earlier this week
    7. 5μL pTXB1(His) + BSA ligation from yesterday
    8. 5μL pTXB1 + BSA ligation from yesterday
    9. -12 --
      • the gel-purified digested vector floated away, so there is still a lot of ethanol in the solution.
    • EtBr stain

Results

Plates

Analytical gel

  • Not surprising that nothing is visible:
    • The double-digested pTXB1 mostly floated away.
    • The ligation reaction lane has about 150ng total [DNA] (the comparable bands in the ladder have ~300ng). This might be visible, but, if the estimations of [DNA] are off, then it might not be visible.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Kathryn_Muratore/Notebook/AU_CHEM-570_lab_prep/2011/06/23&oldid=518897"