User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/26: Difference between revisions
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| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
|- | |- | ||
| 1. | | 1. L34-032 || 0.167 || 1.863 || 167.3 | ||
|- | |- | ||
| 2. | | 2. L34-040 || 0.026 || 1.908 || 26.4 | ||
|- | |||
| 3. L34-094 || 0.032 || 1.901 || 32.4 | |||
|- | |||
| 4. L34-096 || 0.142 || 1.859 || 142.4 | |||
|} | |} | ||
* Sequencing | * Sequencing: Order 10437 | ||
# L34-032, P163 | |||
# L34-040 " | |||
# L34-094 " | |||
# L34-096 " | |||
---- | |||
'''Cultures - CRISPR library (round 2)''' | |||
* Re-grow cultures for clones with failed sequencing runs | |||
* From DNASU Order 10333 | |||
** Note: DNASU sampling from the 96-well block is top to bottom, then left to right | |||
** A1 = G34-001, A2 = G34-002, A3 = G34-003, etc. | |||
* Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling | |||
* Scrape bacteria from the original spot agar plates; grow as 5 mL culture for miniprep (overnight) | |||
Clone, well (agar spot position), phred score | |||
# G34-024, H3, 746 | |||
# G34-067, C9, 444 | |||
# G34-079, G10, 888 | |||
Revision as of 17:05, 26 August 2015
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08/26/15
Minipreps - CRISPR library
Cultures - CRISPR library (round 2)
Clone, well (agar spot position), phred score
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