User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/25

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08/25/15

  • Cultures - CRISPR library 5 mL cultures



CRISPR library 5 mL cultures

  • Re-grow cultures for clones with failed sequencing runs
  • From DNASU Order 10294
    • Note: DNASU sampling from the 96-well block is top to bottom, then left to right
    • A1 = L34-001, A2 = L34-002, A3 = L34-003, etc.
  • Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling
  • Scrape bacteria from the original spot agar plates; grow as 5 mL culture for miniprep (overnight)

Clone, well (agar spot position), phred score

  1. L34-003, C1, 313
  2. L34-015, G2, 281
  3. L34-027, C4, 399
  4. L34-032, H4, 339
  5. L34-040, H5, 494
  6. L34-050, B7, 303
  7. L34-055, G7, 563
  8. L34-071, G9, 237
  9. L34-075, C10, 300
  10. L34-078, F10, 647
  11. L34-080, G10, 495
  12. L34-082, B11, 308
  13. L34-084, D11, 498
  14. L34-093, E12, 8
  15. L34-094, F12, 611
  16. L34-096, H12, 814


RESULTS (08/26/15)

  • Only four clones grew
  1. L34-032
  2. L34-040
  3. L34-094
  4. L34-096



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