User:Karmella Haynes/Notebook/BioBrick cloning/2015/06/24

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06/24/15

  • Cas-tone - minipreps, digests


Cas-tone - minipreps, digests

  • Minipreps
    • Sigma GenElute kit, elute w/ 75 μL elution sln.
  • Digests
    • Check with #/# digests
Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.




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