User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/13

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(12/13/12)
Current revision (17:41, 17 December 2012) (view source)
(12/13/12)
 
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| bgcolor=#cfcfcf | Mix (x9)  
| bgcolor=#cfcfcf | Mix (x9)  
| rowspan="7" | <u>Expected:</u><br>1-8. KAH111/C-His = 3600, 1123<br>Empty C-His = 3600<br>
| rowspan="7" | <u>Expected:</u><br>1-8. KAH111/C-His = 3600, 1123<br>Empty C-His = 3600<br>
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| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
+
| rowspan="7" | <font color=red>Blank gel! (only ladder)</font><br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA(plasmid) || 2.0 || ---
| DNA(plasmid) || 2.0 || ---

Current revision

Karmella's BioBrick Cloning Main project page
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12/13/12

  • Mnipreps & Digests: KAH111/C-His clones



Minipreps

  • Check with E/P digests
Reagent Volume Mix (x9) Expected:
1-8. KAH111/C-His = 3600, 1123
Empty C-His = 3600
Blank gel! (only ladder)
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 2.0 ---
10X buffer 1.5 13.5
EcoRI 1.0 9.0
PstI 1.0 9.0
dH2O 9.5 85.5
  15 μL

--> 37°C/ ~15 min.



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