User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/13: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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| bgcolor=#cfcfcf | Mix (x9)  
| bgcolor=#cfcfcf | Mix (x9)  
| rowspan="7" | <u>Expected:</u><br>1-8. KAH111/C-His = 3600, 1123<br>Empty C-His = 3600<br>
| rowspan="7" | <u>Expected:</u><br>1-8. KAH111/C-His = 3600, 1123<br>Empty C-His = 3600<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | <font color=red>Blank gel! (only ladder)</font><br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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| DNA(plasmid) || 2.0 || ---
| DNA(plasmid) || 2.0 || ---

Latest revision as of 22:19, 26 September 2017

Karmella's BioBrick Cloning Main project page
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12/13/12

  • Mnipreps & Digests: KAH111/C-His clones


Minipreps

  • Check with E/P digests
Reagent Volume Mix (x9) Expected:
1-8. KAH111/C-His = 3600, 1123
Empty C-His = 3600
 Blank gel! (only ladder)
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 2.0 ---
10X buffer 1.5 13.5
EcoRI 1.0 9.0
PstI 1.0 9.0
dH2O 9.5 85.5
  15 μL

--> 37°C/ ~15 min.



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