User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22: Difference between revisions
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==OBJECTIVES== | ==OBJECTIVES== | ||
* Prepare more microsphere samples expanding upon new previous method developed (new modifications) | * Prepare more microsphere samples expanding upon new previous method developed (new modifications) | ||
==PVOH 130K Prepared Microsphere Samples & Dye Preparations 2== | |||
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13''' | |||
'''1μM Rhodamine 6G Dye Concentration (90:10)''' | |||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 10mL = (92μM)V<sub>2</sub> V<sub>2</sub> = 109μL | |||
'''1μM Rhodamine 6G Dye Concentration (50:50)''' | |||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 10mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 61μL | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Clay Selection''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)''' | |||
|- | |||
| 50:50||NaMT||0.02530||0.02530||40||165||61 | |||
|- | |||
| 50:50||Laponite||0.02590||0.02500||40||165||61 | |||
|- | |||
| 90:10||NaMT||0.04140||0.01110||40||92||109 | |||
|- | |||
| 90:10||Laponite||0.04470||0.01100||40||92||109 | |||
|- | |||
| 50:50||110% CEC Laponite w/ DMHXLBR||0.02550||0.02500||40||165||61 | |||
|- | |||
| 90:10||110% CEC Laponite w/ DMHXLBR||0.04230||0.01150||40||92||109 | |||
|- | |||
| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.02540||0.02500||40||165||61 | |||
|- | |||
| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.04010||0.01050||40||92||109 | |||
|} | |||
==Notes== | |||
* Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include: | |||
# 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance | |||
# 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH | |||
* Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres | |||
==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4== | ==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4== |
Revision as of 13:37, 24 March 2013
PVOH Research | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OBJECTIVES
PVOH 130K Prepared Microsphere Samples & Dye Preparations 2RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13 1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Notes
# 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13 1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
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