User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/18

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Friday 18th June 2010

Today I performed 2 double restrictions:

  • GFP cut with XbaI and PstI
  • Plasmid 18 cut with EcoRI and PstI

as follows:

For GFP
Reactive...........1x [ul]
DNA................25
BSA..................1
Buffer2............4
H2O................7
PstI................1.5
XbaI...............1.5
Total...............40


  • For plasmid 18

Reactive...............1x [ul]
DNA....................10
BSA......................1
Buffer2................3
H2O...................13
EcoRI-HF.............1.5
PstI......................1.5
Total..................30

I left the digestions in the incubator overnight.




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