User:Jarle Pahr/Buffers

Qiagen EB buffer: 10 mM Tris-HCl, pH 8.5.

See also http://people.mbi.ucla.edu/sumchan/qiagenbuffer.html

Buffer	Composition	Use	Storage	Hazardous chemicals?	Comments
TAE	40mM Tris, 20mM acetic acid, and 1mM EDTA	Electrophoresis	Room temperature
Qiagen EB	10 mM Tris-HCl, pH 8.5	DNA elution and storage	Room temperature	-
TE (Tris-EDTA)	10 mM Tris-HCl, pH 8.5 + 10 mM Tris-HCl, pH 8 + 1 mM EDTA	DNA elution and storage	?	-	EDTA inhbitis nucleases and also inhibits sequencing reactions.
NEBuffer 1	10mM Bis-Tris-Propane-HCl, 10mM MgCl2,1mM DTT,pH 7.0@25°C	Restriction digestion	-20C	-	CIP shows 50 % activity in NEBuffer 1, compared with 100 % activity in NEBuffers 2-4.
NEBuffer 2	50mM NaCl,10mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C	Restriction digestion	-20C	-
NEBuffer 3	100mM NaCl,50mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C	Restriction digestion	-20C	-
NEBuffer 4	50mM Potassium Acetate,20mM Tris-acetate,10mM Magnesium Acetate,1mM DTT,pH 7.9@25°C	Restriction digestion	-20C	-
Phosphate Buffered Saline (PBS)			8 g/L NaCl, 0.2 g/L KCl, 1.44 g/L Na2HPO4, 0.22 g/L KH2PO4. Adjust pH to 7.4 with HCl.	http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8247.full?text_only=true

Transformation and Storage Solution (TSS):

LB with 10 wt/vol % polyethylene glycol (PEG), 5 % v/v DMSO, 50 mM Mg2+, pH 6.5.

HEPES:

http://en.wikipedia.org/wiki/HEPES

MOPS:

http://en.wikipedia.org/wiki/MOPS