User:Jamie Nunziata/Notebook/Protease Research/2015/09/23: Difference between revisions
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First was creating a 5050µg/mL lysozyme solution by combining 0.1039g with 5mL of Tris buffer in a volumetric flask. Using the equation M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub>, the amount Bradford Assay, lysozyme solution, protein assay reagent, and Tris buffer buffer needed for our various solutions. Those quantities can be found below: | First was creating a 5050µg/mL lysozyme solution by combining 0.1039g with 5mL of Tris buffer in a volumetric flask. Using the equation M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub>, the amount Bradford Assay, lysozyme solution, protein assay reagent, and Tris buffer buffer needed for our various solutions. Those quantities can be found below: | ||
[[Image:Nunziata_BradfordAssayConcentrations_9_23.png]] | [[Image:Nunziata_BradfordAssayConcentrations_9_23.png]] | ||
Revision as of 16:28, 1 October 2015
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ObjectiveThe objective for today's lab work is to create variety lysozyme solutions from 1-10µg/mL and to analyze them using a Bradford assay in the UV Vis spectronomer
ProcedureWe loosely based our procedure this experiment from the one Dr. Hartings had in his lab notebook. First was creating a 5050µg/mL lysozyme solution by combining 0.1039g with 5mL of Tris buffer in a volumetric flask. Using the equation M1V1=M2V2, the amount Bradford Assay, lysozyme solution, protein assay reagent, and Tris buffer buffer needed for our various solutions. Those quantities can be found below:
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