User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/16: Difference between revisions
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== | ==Objective== | ||
* | * Synthesize HgS-BSA QDs at 40°C |'''JCS 2''' | ||
* Obtain UVVis and fluorescence data for JCS 1 | |||
==Protocol== | |||
#Making Stock Solutions | |||
##Same stock solutions from [[User:James_C._Schwabacher/Notebook/Protein-Templated_Quantum_Dots/2014/09/09|last week]] | |||
#Synthesis | |||
##Added 5 mL of 15mg/mL BSA from Eleni's stock solution to 5 mL of the HgNO<sub>3</sub> in HPLC water | |||
## stirred vigorously while adding 2 drops of 1 M NaOH to reach pH ~9 | |||
## capped to sit and react for ~12 hours | |||
==Notes== | |||
*The HgNO<sub>3</sub> solution was sonicated for 15 minutes prior to use. | |||
*The pH test trip was slightly darker than the coding for a pH 9 (~pH 10), like the last synthesis. This time only 50 μL of 1M NaOH was added. | |||
*The heating mantle was set to 11. As of this afternoon, this corresponded to about 41°C | |||
Revision as of 17:26, 16 September 2014
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