User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/16

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Objective

  • Synthesize HgS-BSA QDs at 40°C |JCS 2
  • Obtain UVVis and fluorescence data for JCS 1

Protocol

  1. Making Stock Solutions
    1. Same stock solutions from last week
  2. Synthesis
    1. Added 5 mL of 15mg/mL BSA from Eleni's stock solution to 5 mL of the HgNO3 in HPLC water
    2. stirred vigorously while adding 2 drops of 1 M NaOH to reach pH ~9
    3. capped to sit and react for ~12 hours

Notes

  • The HgNO3 solution was sonicated for 15 minutes prior to use.
  • The pH test trip was slightly darker than the coding for a pH 9 (~pH 10), like the last synthesis. This time only 50 μL of 1M NaOH was added.
  • The heating mantle was set to 11. As of this afternoon, this corresponded to about 41°C



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