User:James C. Schwabacher/Notebook/CHEM-571/2014/03/26: Difference between revisions
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Learn how to maintain an OpenWetWare Notebook. | Learn how to maintain an OpenWetWare Notebook. | ||
== | ==Stock Solution Prep== | ||
# | The solutions created for 78%BSA by mass with L-Cysteine synthesis: | ||
# 250mL of 20mM L-cysteine | |||
## FW 121.16g/mol | |||
## measure out: 0.6058g | |||
## measured: 0.6072g | |||
## actual conc: 20.05mM | |||
# 50mL of 100mM ZnSO<sub>4</sub> | |||
## FW 287.56g/mol | |||
## measure out: 1.4378g | |||
## measured: 1.4399 g | |||
## actual conc: 100.1 mM | |||
# 50mL of 10mM MnCl<sub>2</sub> | |||
## FW 197.9g/mol | |||
## measure out: 0.09895g | |||
## measured: 0.0987g | |||
## actual conc: 9.98 mM | |||
# 10mL 1M NaOH | |||
## FW 40.00g/mol | |||
## 0.4284g | |||
## Final concentration a bit over 1M check pH carefully when adjusting! | |||
# 25mL 100mM Na<sub>2</sub>S | |||
## FW 240.18g/mol | |||
## measure out: 0.600g | |||
## measured: 0.610g | |||
## actual conc: 101.6 mM | |||
The protocol followed was the one used previously for the 78% BSA-L-Cysteine QDs | |||
==Data== | ==Data== |
Revision as of 10:52, 26 March 2014
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ObjectiveLearn how to maintain an OpenWetWare Notebook. Stock Solution PrepThe solutions created for 78%BSA by mass with L-Cysteine synthesis:
The protocol followed was the one used previously for the 78% BSA-L-Cysteine QDs Data
NotesThis area is for any observations or conclusions that you would like to note.
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