User:James C. Schwabacher/Notebook/CHEM-571/2014/03/26

Biomaterials Design Lab

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Objective Repeat the 78% BSA-L-Cysteine Synthesis Continue precipitation of large volume samples Stock Solution Prep The solutions created for 78%BSA by mass with L-Cysteine synthesis: 250mL of 20mM L-cysteine FW 121.16g/mol measure out: 0.6058g measured: 0.6072g actual conc: 20.05mM 50mL of 100mM ZnSO4 FW 287.56g/mol measure out: 1.4378g measured: 1.4399 g actual conc: 100.1 mM 50mL of 10mM MnCl2 FW 197.9g/mol measure out: 0.09895g measured: 0.0987g actual conc: 9.98 mM 10mL 1M NaOH FW 40.00g/mol 0.4284g Final concentration a bit over 1M check pH carefully when adjusting! 25mL 100mM Na2S FW 240.18g/mol measure out: 0.600g measured: 0.610g actual conc: 101.6 mM 25mL .1484 mM BSA The protocol followed was the one used previously for the 78% BSA-L-Cysteine QDs Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.