User:Jacob Esenther/Notebook/581/2014/10/01: Difference between revisions

From OpenWetWare
< User:Jacob Esenther‎ | Notebook‎ | 581‎ | 2014‎ | 10
Jump to navigationJump to search
(fix raw html notebook nav)
 
(4 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 16: Line 16:
<u>Diffusion</u>
<u>Diffusion</u>
# Our group chose to use a PVA/clay film
# Our group chose to use a PVA/clay film
# Michael's group is using a PVA film. Their protocol may be viewed here.
# Michael's group is using a PVA film. Their protocol may be viewed [[User:Michael_S._Bible/Notebook/581/2014/10/01|here]].
# the films were set between the two chambers
# the films were set between the two chambers
# Added liquid to each chamber
# Added liquid to each chamber
## One chamber got DI water
## One chamber got DI water
## The other chamber got 20ppm Malachite green
## The other chamber got 20ppm Malachite green
### Stock solution made by Mary.
### Stock solution made by Mary, see [[User:Mary_Mendoza/Notebook/CHEM_581:_Experimental_Chemistry_I/2014/10/01|here]].
# At the 5 minute mark, 200uL was taken from each chamber and placed in an eppy tube
# At the 5 minute mark, 200uL was taken from each chamber and placed in an eppy tube
# Measure the UV-Vis spectrum for both samples using a small volume cuvette. Return each to the chamber it originally came from
# Measure the UV-Vis spectrum for both samples using a small volume cuvette. Return each to the chamber it originally came from
Line 30: Line 30:
## 2 hours
## 2 hours
## right before you leave lab
## right before you leave lab


==Data==
==Data==
* Mass of Film Piece - 0.8846g
* Mass of Film Piece - 0.8846g
* Width of Film Piece - 0.51mm
* Diameter of Diffusion Tube - 14.41mm


==Notes==
==Notes==

Latest revision as of 00:21, 27 September 2017

Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

Today, we are looking at the diffusion of Malachite Green across a film.

Description

Diffusion

  1. Our group chose to use a PVA/clay film
  2. Michael's group is using a PVA film. Their protocol may be viewed here.
  3. the films were set between the two chambers
  4. Added liquid to each chamber
    1. One chamber got DI water
    2. The other chamber got 20ppm Malachite green
      1. Stock solution made by Mary, see here.
  5. At the 5 minute mark, 200uL was taken from each chamber and placed in an eppy tube
  6. Measure the UV-Vis spectrum for both samples using a small volume cuvette. Return each to the chamber it originally came from
  7. Take measurements at the following times
    1. 15 minutes
    2. 30 minutes
    3. 1 hour
    4. 2 hours
    5. right before you leave lab

Data

  • Mass of Film Piece - 0.8846g
  • Width of Film Piece - 0.51mm
  • Diameter of Diffusion Tube - 14.41mm

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.