User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29: Difference between revisions

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==Entry title==
==29 May 2013==
* Insert content here...
*Seeded GFP/L4440 plates
      -conc. from 10mL LB to 1.5mL
      -blinded for adam: L4440=squiggles, GFP=wiggles
*Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
*was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
*Made M9 solution:
      Per 1 L of M9:              Adjusted for 700mL              Notes:
      -KH2PO4        3g            2.1g                            FisherChemicals F.W.141.96, lot no. 005502 Date:8.07.00
      -Na2HPO4      6g            4.2g                            FisherChemicals F.W. 136.09, lot no. 995072 Date?
      -NaCl          5g            3.5g                            NaCl F.W. 58.44
      -H2O to 1L                  water to 700mL                  MilliQ, prepared by Skyler
      -read below                  700µL MgSO4                      1 M prepared by Lena, aliquoted 29 May 2013
 
      Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
      add 1mL of MgSO4 sterily
           




Revision as of 16:12, 29 May 2013

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29 May 2013

  • Seeded GFP/L4440 plates
     -conc. from 10mL LB to 1.5mL
     -blinded for adam: L4440=squiggles, GFP=wiggles
  • Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
  • was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
  • Made M9 solution:
     Per 1 L of M9:               Adjusted for 700mL               Notes:
     -KH2PO4        3g             2.1g                            FisherChemicals F.W.141.96, lot no. 005502 Date:8.07.00
     -Na2HPO4       6g             4.2g                            FisherChemicals F.W. 136.09, lot no. 995072 Date?
     -NaCl          5g             3.5g                            NaCl F.W. 58.44
     -H2O to 1L                   water to 700mL                   MilliQ, prepared by Skyler
     -read below                  700µL MgSO4                      1 M prepared by Lena, aliquoted 29 May 2013

     Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
     add 1mL of MgSO4 sterily



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