User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/16: Difference between revisions
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== | ==Objectives== | ||
#Run UV-vis for 20 samples with lysozyme as the protein. Full concentration details can be found below under Data. | |||
==Description== | |||
#Test solutions made according to below concentrations under Data. | |||
#On 11/15, solutions were placed into the oven at 80°C for 4 hours. | |||
#UV-vis data was run on each of the 20 samples of different pHs and fructose concentrations. | |||
#After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H<sub>2</sub>O, twice with HCl, and twice more with H<sub>2</sub>O. The cuvette was then dried thoroughly. | |||
==Data== | |||
'''Solution concentrations'''<br> | |||
<u>UV-vis solutions for 11.16.2016</u><br> | |||
[[Image:111616 UVvis Lys Solutions.png.png]]<br> | |||
[[Image:111615 UV Ag Abs Wave Fruc.75.png|thumb|upright=4.5]] <br> | |||
'''Figure 1''' Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose; Ag | |||
[[Image:111615 UV Ag Abs pH Fruc.75.png|thumb|upright=4.5]] <br> | |||
'''Figure 2''' Absorbance at 272 nm at various pH's in 0.75 mM concentration of fructose; Ag | |||
[[Image:111615 UV Ag Abs Wave pH Fruc1.25.png|thumb|upright=4.5]] <br> | |||
'''Figure 3''' Absorbance v wavelength of lysozyme in 1.25 mM concentration of fructose; Ag | |||
[[Image:111615 UV Ag Abs pH Fruc1.25.png|thumb|upright=4.5]] <br> | |||
'''Figure 4''' Absorbance at 280 nm at various pH's in 1.25 mM concentration of fructose; Ag <br> | |||
[[Image:111615 UV Au Abs Wave pH Fruc0.png|thumb|upright=4.5]] <br> | |||
'''Figure 5''' Absorbance v wavelength of lysozyme in 0.0 mM concentration of fructose; Au | |||
[[Image:111615 UV Au Abs pH Fruc0.png|thumb|upright=4.5]] <br> | |||
'''Figure 6''' Absorbance at 527 nm at various pH's in 0.0 mM concentration of fructose; Au | |||
[[Image:111615 UV Au Abs Wave pH Fruc.0625.png|thumb|upright=4.5]] <br> | |||
'''Figure 7''' Absorbance v wavelength of lysozyme in 0.0625 mM concentration of fructose; Au | |||
[[Image:111615 UV Au Abs pH Fruc.0625.png|thumb|upright=4.5]] <br> | |||
'''Figure 8''' Absorbance at 527 nm at various pH's in 0.0625 mM concentration of fructose; Au <br> | |||
==Notes== | |||
All Figures in the above data are baseline and water corrected. <br> | |||
Revision as of 11:52, 30 November 2016
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Objectives
Description
DataSolution concentrations  Figure 1 Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose; Ag  Figure 2 Absorbance at 272 nm at various pH's in 0.75 mM concentration of fructose; Ag  Figure 3 Absorbance v wavelength of lysozyme in 1.25 mM concentration of fructose; Ag  Figure 4 Absorbance at 280 nm at various pH's in 1.25 mM concentration of fructose; Ag  Figure 5 Absorbance v wavelength of lysozyme in 0.0 mM concentration of fructose; Au  Figure 6 Absorbance at 527 nm at various pH's in 0.0 mM concentration of fructose; Au  Figure 7 Absorbance v wavelength of lysozyme in 0.0625 mM concentration of fructose; Au  Figure 8 Absorbance at 527 nm at various pH's in 0.0625 mM concentration of fructose; Au NotesAll Figures in the above data are baseline and water corrected.
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