User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/25: Difference between revisions
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== | ==Objectives== | ||
#Run fluorescence data for lysozyme at pH=7 | |||
#Create stock solutions for use on 10.05.2016 | |||
==Description== | |||
<u>Fluorescence</u> | |||
#Solutions made using measurements below | |||
#Fluorescence data run every 3 minutes for 3 hours | |||
<u>Solutions for 10/05/2016</u><br> | |||
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours. | |||
==Data== | |||
[[Image:100416 Emit vs Time pH 7.png|thumb|upright=5.0]]<br> | |||
'''Figure 1''' Maximum emission data in a solution of pH 7.<br> | |||
[[Image:100416 Int v Time Lys pH 7.png|thumb|upright=5.0]]<br> | |||
'''Figure 2''' Integrated fluorescence data in a solution of pH 7. <br> | |||
[[Image:100416 Full Fluor Lys pH 7.png|thumb|upright=5.0]]<br> | |||
'''Figure 3''' Full emission spectra for important time points: t=0, 15, 33, 105, 180 min. <br> | |||
<u>'''Figure 3''' Explanation</u><br> | |||
#At t=0 min, we see a max emission far to the left of the other emission spectra. | |||
#At t=15 min, this maximum has shifted up and to the right. | |||
#Until t=33 min, the maximum for each spectra decreases until the shown maximum. | |||
#After t=33 min, the maximum begins increasing again. | |||
#At t=105 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away. | |||
#t=180 shows the final full spectra obtained.<br> | |||
<u>UV-vis solutions for 09.14.2016</u><br> | |||
[[Image:AgNP Fruct 0mM Lys UVvis.png]]<br> | |||
'''Table 1''' UV-vis solutions made for [Fructose]=.0mM with silver | |||
[[Image:Image-AgNP Fruct .0625mM Lys UVvis.png.png]]<br> | |||
'''Table 2''' UV-vis solutions made for [Fructose]=.0625mM with silver | |||
Revision as of 10:22, 25 October 2016
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Objectives
DescriptionFluorescence
Solutions for 10/05/2016 DataFigure 1 Maximum emission data in a solution of pH 7. Figure 2 Integrated fluorescence data in a solution of pH 7. Figure 3 Full emission spectra for important time points: t=0, 15, 33, 105, 180 min. Figure 3 Explanation
UV-vis solutions for 09.14.2016
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