User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/18: Difference between revisions
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== | ==Objectives== | ||
#Run UV-vis for 18 samples with lysozyme as the protein. Full concentration details can be found below under Data. | |||
==Description== | |||
#Test solutions made according to below concentrations under Data. | |||
#On 10-12-16, solutions were placed into the oven at 80°C for 4 hours. | |||
#UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations. | |||
#After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H<sub>2</sub>O, twice with HCl, and twice more with H<sub>2</sub>O. The cuvette was then dried thoroughly. | |||
==Data== | |||
<u>UV-vis solutions</u><br> | |||
[[Image:101816 UVvis Conc Fruc .125mM.png]]<br> | |||
'''Table 1''' UV-vis solutions made for [Fructose]=.125mM with silver | |||
[[Image:101816 UVvis Conc Fruc .25mM.png]]<br> | |||
'''Table 2''' UV-vis solutions made for [Fructose]=.25mM with silver | |||
[[Image:101816 Abs v Wave Fruc .125mM.png|thumb|upright=5.0]] <br> | |||
'''Figure 1''' Absorbance v wavelength of lysozyme in 0.125 mM concentration of fructose | |||
[[Image:101816 Abs v pH Fruct .125mM.png|thumb|upright=5.0]] <br> | |||
'''Figure 2''' Absorbance at 279 nm at various pH's in 0.125 mM concentration of fructose | |||
[[Image:101816 Abs v Wave Fruc .25mM.png|thumb|upright=5.0]] <br> | |||
'''Figure 3''' Absorbance v wavelength of lysozyme in 0.25 mM concentration of fructose | |||
[[Image:101816 Abs v pH Fruc .25mM.png|thumb|upright=5.0]] <br> | |||
'''Figure 4''' Absorbance at 279 nm at various pH's in .25 mM concentration of fructose <br> | |||
==Notes== | |||
All Figures in the above data are baseline and water corrected. <br> | |||
After taking the UV-vis samples out of the 80°C oven, pH 11 for both Fructose concentrations showed a precipitate form. pH 12 did not show this precipitate. | |||
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Revision as of 16:01, 25 October 2016
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Objectives
Description
DataUV-vis solutions
Figure 1 Absorbance v wavelength of lysozyme in 0.125 mM concentration of fructose Figure 2 Absorbance at 279 nm at various pH's in 0.125 mM concentration of fructose Figure 3 Absorbance v wavelength of lysozyme in 0.25 mM concentration of fructose Figure 4 Absorbance at 279 nm at various pH's in .25 mM concentration of fructose NotesAll Figures in the above data are baseline and water corrected. |