User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/12: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objectives==
==Objectives==
#Run fluorescence data for lysozyme at pH=7
#Run fluorescence data for lysozyme at pH=8
#Create stock solutions for use on 10.05.2016
#Create stock solutions for use on 10.18.2016


==Description==
==Description==
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#Fluorescence data run every 3 minutes for 3 hours
#Fluorescence data run every 3 minutes for 3 hours


<u>Solutions for 10/05/2016</u><br>
<u>Solutions for 10/18/2016</u><br>
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.


==Data==
==Data==


[[Image:100416 Emit vs Time pH 7.png|thumb|upright=5.0]]<br>
[[Image:101216 Lys Emis v time pH 8.png|thumb|upright=5.0]]<br>
'''Figure 1''' Maximum emission data in a solution of pH 7.<br>
'''Figure 1''' Maximum emission data in a solution of pH 8.<br>


[[Image:100416 Int v Time Lys pH 7.png|thumb|upright=5.0]]<br>
[[Image:101216 Lys Int v time pH 8.png|thumb|upright=5.0]]<br>
'''Figure 2''' Integrated fluorescence data in a solution of pH 7. <br>
'''Figure 2''' Integrated fluorescence data in a solution of pH 8. <br>


[[Image:100416 Full Fluor Lys pH 7.png|thumb|upright=5.0]]<br>
[[Image:101216 Lys Fluor Summary pH 8.png|thumb|upright=5.0]]<br>
'''Figure 3''' Full emission spectra for important time points: t=0, 15, 33, 105, 180 min. <br>
'''Figure 3''' Full emission spectra for important time points: t=0, 3, 21, 39, 159, 180 min. <br>


<u>'''Figure 3''' Explanation</u><br>
<u>'''Figure 3''' Explanation</u><br>
#At t=0 min, we see a max emission far to the left of the other emission spectra.  
#At t=0 min, we see a max emission far to the left of the other emission spectra.  
#At t=15 min, this maximum has shifted up and to the right.  
#At t=3 min, this maximum has shifted up and to the right.  
#Until t=33 min, the maximum for each spectra decreases until the shown maximum.  
#Until t=39 min, the maximum for each spectra decreases until the shown maximum.  
#After t=33 min, the maximum begins increasing again.  
#After t=39 min, the maximum begins increasing again.  
#At t=105 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.  
#At t=159 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.  
#t=180 shows the final full spectra obtained.<br>
#t=180 shows the final full spectra obtained.<br>


<u>UV-vis solutions for 09.14.2016</u><br>
<u>UV-vis solutions for 10.18.2016</u><br>
[[Image:AgNP Fruct 0mM Lys UVvis.png]]<br>
[[Image:101816 UVvis Conc Fruc .125mM.png]]<br>
'''Table 1''' UV-vis solutions made for [Fructose]=.0mM with silver  
'''Table 1''' UV-vis solutions made for [Fructose]=.125mM with silver  


[[Image:Image-AgNP Fruct .0625mM Lys UVvis.png.png]]<br>
[[Image:101816 UVvis Conc Fruc .25mM.png]]<br>
'''Table 2''' UV-vis solutions made for [Fructose]=.0625mM with silver
'''Table 2''' UV-vis solutions made for [Fructose]=.25mM with silver


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Latest revision as of 02:05, 27 September 2017

Project name Main project page
Previous entry      Next entry

Objectives

  1. Run fluorescence data for lysozyme at pH=8
  2. Create stock solutions for use on 10.18.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 3 minutes for 3 hours

Solutions for 10/18/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data


Figure 1 Maximum emission data in a solution of pH 8.


Figure 2 Integrated fluorescence data in a solution of pH 8.


Figure 3 Full emission spectra for important time points: t=0, 3, 21, 39, 159, 180 min.

Figure 3 Explanation

  1. At t=0 min, we see a max emission far to the left of the other emission spectra.
  2. At t=3 min, this maximum has shifted up and to the right.
  3. Until t=39 min, the maximum for each spectra decreases until the shown maximum.
  4. After t=39 min, the maximum begins increasing again.
  5. At t=159 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
  6. t=180 shows the final full spectra obtained.

UV-vis solutions for 10.18.2016

Table 1 UV-vis solutions made for [Fructose]=.125mM with silver


Table 2 UV-vis solutions made for [Fructose]=.25mM with silver


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