User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/21

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Objectives

  1. Run fluorescence data for lysozyme at pH=4
  2. Create stock solutions for use on 09.27.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 5 minutes for 3 hours

Solutions for 09/27/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data

Fluorescence

  1. Solution Measurements
    1. Lysosome Stock Solution
      1. concentration = 225 μM
    2. Gold Stock Solution
      1. concentration = 3.73 mM
    3. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 6
      1. 223 μL AuCl3
      2. 3 μL 1M HCl
      3. 167 μL Lysozyme
      4. 2607 μL Water
  2. Fluorescence Summary Graphs

[[|thumb|upright=4.0]]
Figure 1 Maximum emission data of tryptophan in a solution of pH 6

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Figure 2 Integrated fluorescence data of tryptophan in a solution of pH 6

[[|thumb|upright=4.0]]
Figure 3 Full emission spectra for selected time points. Data shows every 5 minutes for the first 30 minutes followed by every 30 minutes until the 180 minute mark.

UV-vis solutions for 09.27.2016

Table 1 UV-vis solutions made for [Fructose]=.75mM


Table 2 UV-vis solutions made for [Fructose]=1.25mM

Notes


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