User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/02/14: Difference between revisions

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==Objective==
==Objective==
Three reactions with the fluorescent dye were carried out.


==Description==
==Description==
# 50 mM Tris buffer (pH 8.73) was added to 10 mL solutions of gold nanoparticles, purple fibers, and a BSA control containing BSA and HCl until the pH of the solutions was 8.50.
# 2 mg of the dye was measured out and dissolved in 160 μL DMSO.  This was repeated two additional times.
# The dye was added to the reaction mixtures with a pH of 8.50.
# The reaction tubes were covered in foil and allowed to shake for 24 hours at room temperature.


==Data==
==Data==
* Add data and results here...
* When raising the pH of the BSA control, 326 μL of the Tris buffer was needed. (Initial pH = 3.98, final pH = 8.50)
* When raising the pH of the purple fibers, 1.5 mL of the Tris buffer was used. (Initial pH = 3.17, final pH = 8.51)
* When raising the pH of the purple solution, 550 μL of the Tris buffer was used. (Initial pH = 3.50, final pH = 8.50)


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
* When the dyes were added to the solutions, color changes were observed.
 
* When the dye was added to the purple solution, the color changed from a light purple/pink color to dark purple on the top and fuchsia at the bottom.
 
* When the dye was added to the purple fibers, the solution turned blue.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
* When the dye was added to the control, the reaction turned blue on top and purple on the bottom.  
 
[[Category:Course]]
[[Category:Miscellaneous]]




Revision as of 13:32, 14 February 2012

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Objective

Three reactions with the fluorescent dye were carried out.

Description

  1. 50 mM Tris buffer (pH 8.73) was added to 10 mL solutions of gold nanoparticles, purple fibers, and a BSA control containing BSA and HCl until the pH of the solutions was 8.50.
  2. 2 mg of the dye was measured out and dissolved in 160 μL DMSO. This was repeated two additional times.
  3. The dye was added to the reaction mixtures with a pH of 8.50.
  4. The reaction tubes were covered in foil and allowed to shake for 24 hours at room temperature.

Data

  • When raising the pH of the BSA control, 326 μL of the Tris buffer was needed. (Initial pH = 3.98, final pH = 8.50)
  • When raising the pH of the purple fibers, 1.5 mL of the Tris buffer was used. (Initial pH = 3.17, final pH = 8.51)
  • When raising the pH of the purple solution, 550 μL of the Tris buffer was used. (Initial pH = 3.50, final pH = 8.50)

Notes

  • When the dyes were added to the solutions, color changes were observed.
  • When the dye was added to the purple solution, the color changed from a light purple/pink color to dark purple on the top and fuchsia at the bottom.
  • When the dye was added to the purple fibers, the solution turned blue.
  • When the dye was added to the control, the reaction turned blue on top and purple on the bottom.



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