User:Despaux: Difference between revisions

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{{Smolke_Top}}
{{Smolke_Top}}
==Leo d'Espaux==
==Leo d'Espaux==
Graduate Student<br/>
Graduate Student
[http://openwetware.org/wiki/Smolke Smolke Lab]<br/>
Smolke Lab
Chemical Engineering, MC 210-41<br/>
Stanford Bioengineering
California Institute of Technology<br/>
Pasadena, CA 91125-4100<br/>
t.  626.395.2753<br/>
f.  626.568.8743<br/>
despaux (at) caltech (dot) edu


==Education==
==Education==
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B.S., Chemical Engineering, [http://www.cornell.edu Cornell]
B.S., Chemical Engineering, [http://www.cornell.edu Cornell]
, 2005
, 2005
==Honors and Awards==
Graduate Research Fellowship, National Science Foundation, 2006-present<br/>
Betty and Gordon Moore Fellowship, California Institute of Technology, 2005-present <br/>
Menschel Public Service Fellowship, Cornell University, 2005<br/>
Slayton Evans Research Award, American Chemical Society, 2003<br/>
Alumni Sponsored Research Award, Cornell University, 2003<br/>
Presidential Research Scholar, Cornell University, 2001-2005<br/>
ACS-Xerox Scholar, American Chemical Society, 2001-2005<br/>
Gates Millenium Scholar, Bill and Melinda Gates Foundation, 2001-2006


==Research==
==Research==
My research concerns nucleic acids as adaptive gene regulatory elements in mammalian cells. These molecules are able to detect changes in the cellular environment, e.g. cancerous or infected, and act accordingly. I'm currently designing and testing RNA molecules to efficiently knock down genes in cancerous cells but not in normal cells. Previously I've worked on human genetic evolution, drug delivery, tissue engineering, novel nucleic acids, and macromolecular transport across nuclear pores.
My research concerns nucleic acids as adaptive gene regulatory elements. These molecules are able to detect changes in the cellular environment, e.g. cancerous or infected, and act accordingly. I'm currently designing and testing RNA molecules to efficiently knock down genes in cancerous cells but not in normal cells.  


==Publications==
==Publications==
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# “Controlled assembly of dendrimer like DNA”, Y. Li, Y.D. Tseng, S.Y. Kwon, L. d'Espaux, J.S. Bunch, P.L McEuen, and D. Luo, ''Nature Materials'' '''3''', 38-42, (January, 2004). doi:10.1038/nmat1045 pmid:14704783. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14704783 PubMed]  [http://www.its.caltech.edu/~despaux/papers/Luo-naturematerials2004.pdf]
# “Controlled assembly of dendrimer like DNA”, Y. Li, Y.D. Tseng, S.Y. Kwon, L. d'Espaux, J.S. Bunch, P.L McEuen, and D. Luo, ''Nature Materials'' '''3''', 38-42, (January, 2004). doi:10.1038/nmat1045 pmid:14704783. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14704783 PubMed]  [http://www.its.caltech.edu/~despaux/papers/Luo-naturematerials2004.pdf]
# “Honeycomb-shaped DNA”,  L. d’Espaux, and D. Luo, ''Proceedings of the 18th Annual Cornell Undergraduate Research Board Forum'', 69 (March, 2002)
# “Honeycomb-shaped DNA”,  L. d’Espaux, and D. Luo, ''Proceedings of the 18th Annual Cornell Undergraduate Research Board Forum'', 69 (March, 2002)
==Protocols==
Here is a list of protocols I have compiled. Please send me an email with any comments or suggestions. I'll put these somewhere more accessible later.
====Protein====
====Mammalian====
#[[Despaux:Ma_FlowCyCompensation|Compensation for two fluorescent proteins in Quanta flow cytometer]]
#[[Despaux:Ma_Mycoplasma|Checking for mycoplasma contamination with PCR]]
====Bacteria====
[http://www.its.caltech.edu/~despaux/protocols/Ba001_Plasmid%20DNA%20Recovery.doc Plasmid prep with alkaline lysis]
[http://www.its.caltech.edu/~despaux/protocols/Ba002_Electroporation.doc Electroporation]
[http://www.its.caltech.edu/~despaux/protocols/En001_Polymerase%20Chain%20Reaction.doc PCR]
[http://www.its.caltech.edu/~despaux/protocols/En002_digestion.doc Restriction endonuclease digestion]
[http://www.its.caltech.edu/~despaux/protocols/En003_Ligation.doc Ligation with T4 Ligase]
[http://www.its.caltech.edu/~despaux/protocols/En004_Klenow%20Polymerization.doc Klenow polymerization]


==Miscellaneous==
==Miscellaneous==

Revision as of 21:15, 4 March 2009

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Leo d'Espaux

Graduate Student Smolke Lab Stanford Bioengineering

Education

Ph.D. Candidate, Chemical Engineering, Caltech , 2007-present
M.S., Chemical Engineering, Caltech , 2007
B.S., Chemical Engineering, Cornell , 2005

Research

My research concerns nucleic acids as adaptive gene regulatory elements. These molecules are able to detect changes in the cellular environment, e.g. cancerous or infected, and act accordingly. I'm currently designing and testing RNA molecules to efficiently knock down genes in cancerous cells but not in normal cells.

Publications

  1. “Controlled assembly of dendrimer like DNA”, Y. Li, Y.D. Tseng, S.Y. Kwon, L. d'Espaux, J.S. Bunch, P.L McEuen, and D. Luo, Nature Materials 3, 38-42, (January, 2004). doi:10.1038/nmat1045 pmid:14704783. PubMed [1]
  2. “Honeycomb-shaped DNA”, L. d’Espaux, and D. Luo, Proceedings of the 18th Annual Cornell Undergraduate Research Board Forum, 69 (March, 2002)

Miscellaneous

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