User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/08: Difference between revisions
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* Get figures together for Australia report | * Get figures together for Australia report | ||
* Prepare Earth Alive figures | * Prepare Earth Alive figures | ||
* Since the Oomycete gradient PCR didn't appear to say the problem was temperature (see below), I'm gonna try a nested approach to get amplifiable sequence for oomycete TRFLP in samples where there isn't much. So I used the DC6 primer in combo with OomR plus 5x25 ul of MgCl per 50 ul reaction. 35 cycles and 62 degree annealing. Will take 1 ul of this and apply it to OomF and OomR reaction, run under same conditions for a total of 70 cycles of amplification. Let see on gels tommorow how well this approach works. | |||
[[Image:August8,2013Gel.tif]] | [[Image:August8,2013Gel.tif]] | ||
Revision as of 11:33, 8 August 2013
 Applied Soil Microbial Ecology
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Remains of the Day
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