User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/02/05

Remains of the Day

• Interviewed intern candidates most of the day
• Did NifH primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and Klebsiella pneumonii as a positive control, E. coli as negative, Methylobacterial cells as test and corn soil DNA as a test.
• Did Oomycete TRFLP primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and infected arrugula rhizosphere as a positive control, and Alliston potato soil DNA as a test.
• Autoclaved LGI broth for use in plate incubation test tommorow.
• Did PCR for fungal trflp of California soils that Joseph extracted since he's been having a hard time doing it.