User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/23: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PcTF breast cancer</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PcTF breast cancer</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Summary==
==Summary==


Summarize what happened today. Put methods, data, and next steps in sections below.
Transfecting BT-549 cells in 6-well plate using the optimized Lipofectamine LTX protocol, viewable [http://openwetware.org/wiki/Image:Lipofectamine_ltx_and_plus_protocol_v2.0.pdf here].


==Methods==
Used 2.5 µg of pMaxGFP plasmid per well.
Treatment plan:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Well no.'''
| align="center" style="background:#f0f0f0;"|'''pMaxGFP (µg)'''
| align="center" style="background:#f0f0f0;"|'''LTX Reagent (µL)'''
|-
| 1||0||0
|-
| 2||2.5||6
|-
| 3||2.5||9
|-
| 4||2.5||12
|-
| 5||2.5||15
|-
| 6||0||15
|}
Wells 1 and 6 are negative controls. Well 1 is no DNA, no LTX. Well 6 is no DNA.
Cells are at 100% confluency, in 2 mL of BT-549 medium (no antibiotic). Overgrowth may impair transfection efficiency somewhat.


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Latest revision as of 01:21, 27 September 2017

PcTF breast cancer Main project page
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Summary

Transfecting BT-549 cells in 6-well plate using the optimized Lipofectamine LTX protocol, viewable here.

Methods

Used 2.5 µg of pMaxGFP plasmid per well.

Treatment plan:

Well no. pMaxGFP (µg) LTX Reagent (µL)
1 0 0
2 2.5 6
3 2.5 9
4 2.5 12
5 2.5 15
6 0 15

Wells 1 and 6 are negative controls. Well 1 is no DNA, no LTX. Well 6 is no DNA.

Cells are at 100% confluency, in 2 mL of BT-549 medium (no antibiotic). Overgrowth may impair transfection efficiency somewhat.


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