User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/12

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Summary

2 days after transfection, I imaged the breast cancer cells, harvested them, and analyzed their transfection rates using flow cytometry.

Turns out the cells don't do so well after 2 days. Probably going to focus on trying to optimize for lipofectamine transfection, or create a stable transfected cell line.

Neon Transfection Results

BT474:

Sample ID Well no. Pulse Voltage Pulse width Pulse no. Transfection Efficiency Recovered (cells/µL) Cell Recovery (normalized to control)
1A1---0.51%5,827100.00%
2A29253022.20%2,62445.03%
3A39503024.05%83314.30%
4A49753024.40%59210.16%
5B110003024.93%70212.05%
6B210253025.87%4367.48%
7B310503025.44%3916.71%
8B410753027.09%2854.89%
9C111003029.45%64111.00%
10C212501033.39%5459.35%
11C313001036.21%2834.86%
12C413501035.77%1843.16%

BT549:

Sample ID Well no. Pulse Voltage Pulse width Pulse no. Transfection Efficiency Recovered (cells/µL) Cell Recovery (normalized to control)
1A1---1.09%487100.00%
2A213502018.33%81.64%
3A314002012.00%51.03%
4A414502015.56%20.41%
5B1110020210.71%61.23%
6B211502029.52%40.82%
7B3120020233.33%20.41%
8B412502020.00%10.21%
9C1110030113.64%40.82%
10C2115030113.33%61.23%
11C312003018.77%61.23%
12C4125030116.67%30.62%



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