Summary
2 days after transfection, I imaged the breast cancer cells, harvested them, and analyzed their transfection rates using flow cytometry.
Turns out the cells don't do so well after 2 days. Probably going to focus on trying to optimize for lipofectamine transfection, or create a stable transfected cell line.
Neon Transfection Results
BT474:
| Sample ID
|
Well no.
|
Pulse Voltage
|
Pulse width
|
Pulse no.
|
Transfection Efficiency
|
Recovered (cells/µL)
|
Cell Recovery (normalized to control)
|
| 1 |
A1 |
- |
- |
- |
0.51% |
5,827 |
100.00%
|
| 2 |
A2 |
925 |
30 |
2 |
2.20% |
2,624 |
45.03%
|
| 3 |
A3 |
950 |
30 |
2 |
4.05% |
833 |
14.30%
|
| 4 |
A4 |
975 |
30 |
2 |
4.40% |
592 |
10.16%
|
| 5 |
B1 |
1000 |
30 |
2 |
4.93% |
702 |
12.05%
|
| 6 |
B2 |
1025 |
30 |
2 |
5.87% |
436 |
7.48%
|
| 7 |
B3 |
1050 |
30 |
2 |
5.44% |
391 |
6.71%
|
| 8 |
B4 |
1075 |
30 |
2 |
7.09% |
285 |
4.89%
|
| 9 |
C1 |
1100 |
30 |
2 |
9.45% |
641 |
11.00%
|
| 10 |
C2 |
1250 |
10 |
3 |
3.39% |
545 |
9.35%
|
| 11 |
C3 |
1300 |
10 |
3 |
6.21% |
283 |
4.86%
|
| 12 |
C4 |
1350 |
10 |
3 |
5.77% |
184 |
3.16%
|
BT549:
| Sample ID
|
Well no.
|
Pulse Voltage
|
Pulse width
|
Pulse no.
|
Transfection Efficiency
|
Recovered (cells/µL)
|
Cell Recovery (normalized to control)
|
| 1 |
A1 |
- |
- |
- |
1.09% |
487 |
100.00%
|
| 2 |
A2 |
1350 |
20 |
1 |
8.33% |
8 |
1.64%
|
| 3 |
A3 |
1400 |
20 |
1 |
2.00% |
5 |
1.03%
|
| 4 |
A4 |
1450 |
20 |
1 |
5.56% |
2 |
0.41%
|
| 5 |
B1 |
1100 |
20 |
2 |
10.71% |
6 |
1.23%
|
| 6 |
B2 |
1150 |
20 |
2 |
9.52% |
4 |
0.82%
|
| 7 |
B3 |
1200 |
20 |
2 |
33.33% |
2 |
0.41%
|
| 8 |
B4 |
1250 |
20 |
2 |
0.00% |
1 |
0.21%
|
| 9 |
C1 |
1100 |
30 |
1 |
13.64% |
4 |
0.82%
|
| 10 |
C2 |
1150 |
30 |
1 |
13.33% |
6 |
1.23%
|
| 11 |
C3 |
1200 |
30 |
1 |
8.77% |
6 |
1.23%
|
| 12 |
C4 |
1250 |
30 |
1 |
16.67% |
3 |
0.62%
|
|
PcTF breast cancer
Main project page
Previous entry Next entry
