User:Daniel Goodman/Notebook/Cluzel/2010/04/02: Difference between revisions
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== | ==Printing spots on glass slide using micrometer stage== | ||
* | * printed from 10.5-11.1 Y axis | ||
* printing produces reproducible spots, smaller than chip width (need to compute exact diameter) | |||
* printing from higher up might reduce spot size, need to check | |||
* printing repeatedly might reduce spot size | |||
* need to quantify OD:cells per area ratio (might change with stationary vs exponential | |||
* phase and 1 gfp image saved in 4-2 dir on titan | |||
* will transfer to agarose later today: saved slide in petri dish at 12:15 pm | |||
==Agarose== | |||
* made 1 ml 4% agarose at 12:20 pm | |||
==Cells== | |||
* grew 1 ml cells with 1 col gfp cells (around 11:45 am); forgot to add amp (1 ul), did so at 12:22 pm | |||
Latest revision as of 09:22, 2 April 2010
Cluzel Lab Notebook Daniel Goodman |
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Printing spots on glass slide using micrometer stage
Agarose
Cells
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