User:Daniel Goodman/Notebook/Cluzel/2010/03/29: Difference between revisions
From OpenWetWare
(Autocreate 2010/03/29 Entry for User:Daniel_Goodman/Notebook/Cluzel) |
|||
Line 1: | Line 1: | ||
{{User:Daniel_Goodman/Notebook/Cluzel/Template:Entry_Base}} | {{User:Daniel_Goodman/Notebook/Cluzel/Template:Entry_Base}} | ||
== | ==Prepare for Afternoon/Evening== | ||
===Agarose=== | |||
4% agarose, 4.5 ml, 0.18 mg low melt agarose | |||
heat at 80 degrees | |||
(put in heat bath at 1:00) | |||
===Cells=== | |||
Put one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm) | |||
===Chips=== | |||
Clean chips in hot water DI bath, 80 degrees | |||
(put in heat bath at 1:00) | |||
===Strains=== | |||
Aliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight | |||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 10:02, 29 March 2010
Cluzel Lab Notebook Daniel Goodman |
Main project page Previous entry Next entry<sitesearch>title=Search this Project</sitesearch> | |
Prepare for Afternoon/EveningAgarose4% agarose, 4.5 ml, 0.18 mg low melt agarose heat at 80 degrees (put in heat bath at 1:00) CellsPut one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm) ChipsClean chips in hot water DI bath, 80 degrees (put in heat bath at 1:00) StrainsAliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight |