User:Daniel Goodman/Notebook/Cluzel/2010/03/29

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Cluzel Lab Notebook
Daniel Goodman
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Prepare for Afternoon/Evening

Agarose

4% agarose, 4.5 ml, 0.18 mg low melt agarose heat at 80 degrees (put in heat bath at 1:00)

Cells

Put one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm)

Chips

Clean chips in hot water DI bath, 80 degrees (put in heat bath at 1:00)

Strains

Aliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight


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