User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/30: Difference between revisions
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Program: Gotaq35cyclo (block A lane5) | |||
* 95°C, 3 min | |||
* 35x[95°C, 10 sec; 48°C, 30 sec; 72°C, 120 sec] | |||
* 72°C, 5 min | |||
* 4°C ∞ | |||
Program: Gotaq35cyclo (block B 1-4, and 6) | |||
* 95°C, 3 min | |||
* 35x[95°C, 10 sec; 64°C, 30 sec; 72°C, 120 sec] | |||
* 72°C, 5 min | |||
* 4°C ∞ | |||
Gel fell and was damaged, PCR will need to be redone. May be better to run a restriction enzyme digest in the future for more accurate results. | Gel fell and was damaged, PCR will need to be redone. May be better to run a restriction enzyme digest in the future for more accurate results. |
Revision as of 11:35, 1 July 2015
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Gel electrophoresis of PCR amp
Program: Gotaq35cyclo (block A lane5)
Program: Gotaq35cyclo (block B 1-4, and 6)
Gel fell and was damaged, PCR will need to be redone. May be better to run a restriction enzyme digest in the future for more accurate results.
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