User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Transforming P300 plasmids==
==Transforming P300 plasmids==


[http://openwetware.org/wiki/Haynes:TransformationPlasmids#Traditional_Method:_Chemically_competent_cells_.2B_plasmid_DNA Transformation protocol]
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
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| align="center" style="background:#f0f0f0;"|'''colonies'''
| align="center" style="background:#f0f0f0;"|'''colonies'''
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| PBS P300||Growth||NA||2.09
| PBS P300||Growth||None||0.00
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| NHA P300||Growth||NA||2.07
| NHA P300||Growth||None||0.00
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| CHA P300||Growth||NA||2.09
| CHA P300||Growth||None||0.00
|}
|}


Samples were transformed using the Heat shock transformation rather than the rapid transformation protocol.  
Plasmids were sent on filter paper and is possible did not remain intact during transit.


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Latest revision as of 01:02, 27 September 2017

Project name Main project page
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PCR amplification of chromatin remodeling

Reagent Volume Expected:
1. ANM5 = 1911,
2. MYB = 159,1764
3. Carm1 = 1827
4. SETD7 = 459,642
5. KMT2E = 1176,4401
6. SETD1A = 420,4704
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 1.0 μL
1X GoTaq 12.5
F primer 1.0
R Primer 1.0
dH2O 9.5
  25 μL

Transforming P300 plasmids

Transformation protocol

Sample ID Expected Observed colonies
PBS P300 Growth None 0.00
NHA P300 Growth None 0.00
CHA P300 Growth None 0.00

Plasmids were sent on filter paper and is possible did not remain intact during transit.


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