User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/21: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/06/21 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->


=Date=
=06/21/2013=


'''List title'''
==Media Preparation with Hygromycine==
# List items
 
=Transgenic Cell Maintenance=
{| {{table}} cellspacing=''7''
|- valign="top"
| '''Cell Type''' || '''Genotype''' || '''Medium''' || '''Supplements<br>(required)''' || '''Maintenance<br>antibiotic(s)'''
|-
| U2OS (adherent) || Flp-in T-REx, hygro-transfected || McCoy's 5A || 10% FBS, 1 % pen/strep || 15 ug/mL blasticidin, '''60''' μg/mL hygromycin
|-
| U2OS (adherent) || Flp-in T-REx, hygro-transfected || McCoy's 5A || 10% FBS, 1 % pen/strep || 15 ug/mL blasticidin, '''200''' μg/mL hygromycin
|-
|}
 
<br>

Revision as of 16:24, 23 July 2013

PcTF Subcloning in E. coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

06/21/2013

Media Preparation with Hygromycine

Transgenic Cell Maintenance

Cell Type Genotype Medium Supplements
(required)		 Maintenance
antibiotic(s)
U2OS (adherent) Flp-in T-REx, hygro-transfected McCoy's 5A 10% FBS, 1 % pen/strep 15 ug/mL blasticidin, 60 μg/mL hygromycin
U2OS (adherent) Flp-in T-REx, hygro-transfected McCoy's 5A 10% FBS, 1 % pen/strep 15 ug/mL blasticidin, 200 μg/mL hygromycin


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/21&oldid=711542"