User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/17
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Prepare Transfected Cells for Hygromycine Treatment
Transfer Transfected Cells from 6 well plate to 10cm cell culture dishes. Target cell concentrations are: 1x106, 1x105, 1x104, 1x103
1x106 Monolayer 100% confluent cells are attached to the bottom of each well
Keep cells in the incubator to reach 90% confluency.