User:Beatriz Gimenez De C./Notebook/571/2014/09/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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** Measure HPLC water, DI water, tap water, and your lysozyme stock solution. | ** Measure HPLC water, DI water, tap water, and your lysozyme stock solution. | ||
==Results== | |||
< | * Ca<sup>2+</sup> calibration | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''CaCl<sub>2</sub> Concentration (μM)''' | |||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | |||
|- | |||
| 5||-44.6 | |||
|- | |||
| 50||8.2 | |||
|- | |||
| 500||39.5 | |||
|- | |||
| 5000||63.5 | |||
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| 50000||86.6 | |||
|} | |} | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Substance''' | |||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | |||
|- | |||
| TAP WATER||51.3 | |||
|- | |||
| HPLC water||-23.0 | |||
|- | |||
| DI water||-51.5 | |||
|- | |||
| Lysozyme 0.5g/L||-19.1 | |||
|} | |||
*Cl<sup>-</sup> calibration | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''NaCl concentration (μM)''' | |||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | |||
|- | |||
| 5||146.5 | |||
|- | |||
| 50||129.6 | |||
|- | |||
| 500||81.3 | |||
|- | |||
| 5000||27.5 | |||
|- | |||
| 50000||-23.9 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Substance''' | |||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | |||
|- | |||
| Tap water||65.5 | |||
|- | |||
| HPLC water||163.5 | |||
|- | |||
| DI water||158.8 | |||
|- | |||
| Lysozyme 0.5g/L||89.4 | |||
|} | |||
* readings with Chloride electrode | |||
** 50 mM CaCl<sub>2</sub> and 50 mM Cu(II)Cl<sub>2</sub> solution gave us a 80.5mV | |||
** 50 mM CaCl<sub>2</sub> and 50 mM KCl solution gave 86.4 mV reading | |||
* Readings with Sodium electrode | |||
** 50mM NaCl and 50 mM NaSO<sub>4</sub> solution gave -27.0 mV | |||
** 50mM NaCl and 50 mM Na<sub>2</sub>HPSO<sub>4</sub><sup>2-</sup> solution gave -30.8 mV | |||
__NOTOC__ | __NOTOC__ |
Latest revision as of 00:21, 27 September 2017
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