User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/15: Difference between revisions
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==Tasks for October 15== | ==Tasks for October 15== | ||
*To run SDS-PAGE | *To run SDS-PAGE | ||
*To perform data analysis of dialysed | *To perform data analysis of dialysed solutions from [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-571_Experimental_Biological_Chemistry/2014/10/14 Oct. 14] | ||
===SDS-PAGE=== | |||
** Warm your samples from [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-571_Experimental_Biological_Chemistry/2014/10/14 Oct. 14] to about 40 °C using heating block | |||
** Transfer solutions to a premade gel | |||
*** Well 1: Precision Plus Protein All Blue Standards | |||
*** Well 3: 0.12 g/L lysozyme Ink A | |||
*** Well 4: 30:1 Lysozyme colloid | |||
*** Well 5: 0.12 g/L Lysozyme | |||
*** Well 6: 0.6 g/L Lysozyme | |||
** Run as previously described. Refer to [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 Dr. Harting's protocol]. | |||
===Determination of molecular weight=== | |||
*By comparison with Precision Plus Protein Standards, the molecular weight of all samples was close to 15 KD, slightly lower, as expected for lysozyme compounds with MW=14,300 KD. | |||
* There was a very small quantity of unknown A and colloid in the gel. The very faint mark observed in well 3 could even be considered to be bleed-over from the 30:1 colloid in well 4. Therefore, electrophoresis was inclonclusive with respect to the molecular weight of unknown A. | |||
[[Image:Electrophoresis gel 151014.jpg]] | |||
[[Image:Precision Plus Protein.jpeg|400x300px|]] | |||
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Latest revision as of 00:26, 27 September 2017
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Tasks for October 15
SDS-PAGE
Determination of molecular weight
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