User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/10: Difference between revisions
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(Autocreate 2014/09/10 Entry for User:Alicia_Rasines_Mazo/Notebook/CHEM-571_Experimental_Biological_Chemistry) |
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==Tasks for September 10== | |||
*To run UV-Vis of Bradford-BSA and Bradford-Lysozyme solutions | |||
*To determine the purity of the protein solutions | |||
===Preparing the solutions for UV-Vis=== | |||
Refer to [http://openwetware.org/wiki/User:Douglas_M._Fox/Notebook/AU_CHEM-571_F2011_Lab_Support/2014/09/10 Dr. Fox's Lab notebook] for full details <br.> | |||
#To prepare 50 mL of a standard saline solution (0.9 wt-%): | |||
#*(50mL)×0.9%=0.45 g of NaCl need to be added | |||
#To prepare 50 mL of a 50 mM Tris 50 mM NaCl solution: | |||
#*(50×10<sup>-3</sup>mol/L)×(0.050L)×(121.14g/mol)=0.3030g of Tris buffer | |||
#*(50×10<sup>-3</sup>mol/L)×(0.050L)×(58.44g/mol)=0.1461g of NaCl | |||
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Revision as of 05:07, 23 September 2014
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Tasks for September 10
Preparing the solutions for UV-VisRefer to Dr. Fox's Lab notebook for full details <br.>
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