User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/09

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Tasks for 9 September 2014

  • Redo Kinetics of Au:Lysozyme at 70ºC
  • Run Fluorescence of amino acid solutions from Sept. 2

Kinetics of AUNP formation

  • Heated a 150 mL of water to 70ºC
  • Transferred 0.5 mL 2.5×10-3M HAuCl4 into 5 culture tubes
  • Added 167 μL from 250 μM lysozyme stock solution to make [Au:Lysozyme]=30
  • Filled up culture tubes to 5 mL total with water
  • Covered test tubes with foil
  • Placed all 5 test tubes in beaker of water from step 1
  • Recorded UV-Vis from 360 to 800 nm every 20 min over a period of 3h

Notes
Fresh 250 mM lysozyme solution was made because the protein solution had deteriorated.

  • (2.5×10-4M)(0.010 L)(14,300 g/mol)=0.0358 g into 10 mL volumetric flask. Make up to mark with distilled water.
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