UA Biophysics:Protocols

ANTIBIOTICS STOCK • Ampicillin • Kanamycin • Chloramphenicol BACTERIA STOCK • Stocks BUFFERS • PBS CHEMICALS • KOH EXTRACTION • Lipid Extration • Carotenoid Extration FLUORESCENCE MICROSCOPY • Tubulin Polymerization with AlexaFluor 488 staining IMMUNOSTAINING • Lymphocytes immunostaining with anti-AlphaTubulin MEDIA • M9 Salts (5x) 1L • M9 (5x) 1L • LB 1L • LB Plates 1L • SD 1L • 1M MgSO4 • 0.1M CaCl2 MICROFLUIDICS • Soft lithography • Master fabrication • Plasma Cleaner Protocol • Cell Insertion Mother Machine • Microfluidics Valves • Microfluidic System for Biofilm growth MOLECULAR BIOLOGY • Q5 Polymerase • Phusion Polymerase PLASMIDS AND RELATED • GenEluteTM HP Plasmid Miniprep Kit • Preparing Chemically Competent Cells • Transforming Chemically Competent • Competent Cells • Transformation of Competent Cells PHAGES • Marking T4 DNA with DAPI • Direct Plating Plaque Assay • Small Drop Plaque Assay System PREPARACIÓN DE LUV Y GUV • LUV • GUV TURBIDOSDOSTATS • Plate turbidostat user manual • Revolver turbidostat with interchanging media Return to UA Biophysics