UA Biophysics:Protocols: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 48: Line 48:
&bull; [[UA Biophysics:Protocols:Master fabrication|Master fabrication]]<br>
&bull; [[UA Biophysics:Protocols:Master fabrication|Master fabrication]]<br>
&bull; [[UA Biophysics:Protocols:Plasma Cleaner Protocol|Plasma Cleaner Protocol]]<br>
&bull; [[UA Biophysics:Protocols:Plasma Cleaner Protocol|Plasma Cleaner Protocol]]<br>
&bull; [[UA Biophysics:Protocols:Cell Insertion Mother Machine|Cell Insertion Mother Machine]]<br>


==MOLECULAR BIOLOGY==
==MOLECULAR BIOLOGY==

Revision as of 14:16, 31 July 2015


ANTIBIOTICS STOCK

• Ampicillin
• Kanamycin
• Chloramphenicol

BACTERIA STOCK

• Stocks

BUFFERS

• PBS


EXTRACTION

• Lipid Extration
• Carotenoid Extration

MEDIA

• M9 Salts (5x) 1L
• M9 (5x) 1L
• LB 1L
• LB Plates 1L
• SD 1L
• 1M MgSO4
• 0.1M CaCl2

MICROFLUIDICS

• Soft lithography
• Master fabrication
• Plasma Cleaner Protocol
• Cell Insertion Mother Machine

MOLECULAR BIOLOGY

• Q5 Polymerase

• Phusion Polymerase

PLASMID

• GenEluteTM HP Plasmid Miniprep Kit
• Preparing Chemically Competent Cells
• Transforming Chemically Competent

PHAGES

• Marking T4 DNA with DAPI

• Direct Plating Plaque Assay

• Small Drop Plaque Assay System

PREPARACIÓN DE LUV Y GUV

• LUV

• GUV

TURBIDOSDOSTATS

• Plate turbidostat user manual
• Revolver turbidostat with interchanging media


Return to UA Biophysics
Retrieved from "https://openwetware.org/mediawiki/index.php?title=UA_Biophysics:Protocols&oldid=895529"