UA Biophysics:Protocols: Difference between revisions

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&bull; [http://openwetware.org/wiki/Transforming_chemically_competent_cells Transforming Chemically Competent]<br>
&bull; [http://openwetware.org/wiki/Transforming_chemically_competent_cells Transforming Chemically Competent]<br>


==PHAGUES==
==PHAGES==


&bull; [[UA Biophysics:Protocols:Marking T4 DNA with DAPI|Marking T4 DNA with DAPI]]<br>
&bull; [[UA Biophysics:Protocols:Marking T4 DNA with DAPI|Marking T4 DNA with DAPI]]<br>

Revision as of 10:44, 31 July 2015


ANTIBIOTICS STOCK

• Ampicillin
• Kanamycin
• Chloramphenicol

BACTERIA STOCK

• Stocks

BUFFERS

• PBS


EXTRACTION

• Lipid Extration
• Carotenoid Extration

MEDIA

• M9 Salts (5x) 1L
• M9 (5x) 1L
• LB 1L
• LB Plates 1L
• SD 1L
• 1M MgSO4
• 0.1M CaCl2

MICROFLUIDICS

• Soft lithography
• Master fabrication
• Plasma Cleaner Protocol

MOLECULAR BIOLOGY

• Q5 Polymerase

• Phusion Polymerase

PLASMID

• GenEluteTM HP Plasmid Miniprep Kit
• Preparing Chemically Competent Cells
• Transforming Chemically Competent

PHAGES

• Marking T4 DNA with DAPI

PREPARACIÓN DE LUV Y GUV

• LUV

• GUV

TURBIDOSDOSTATS

• Plate turbidostat user manual
• Revolver turbidostat with interchanging media


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