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From OpenWetWare
Welcome to your project page!
For each part listed, you should:
- Design oligos to make your part
- Write up proper construction files and put it on the Construction Files page
- Put your oligos on the Oligo Log page
- Put your part sequence on the part document
- Name your parts according to the list here
Circularly Permuted ompX
Source: E. coli MG1655 genomic DNA
Before starting design, you should read this tutorial:
A Guide to Circular Permution
In general, you should clone the bits and pieces out of genomic DNA first, subclone and sequence those bits, then assemble them into full length in a separate step. You can use either SOEing as described in the tutorial to do the assembly, or use standard assembly.
The reference for linker composition of eCPX is: PMID: 18480093
This part encodes a N-terminal display protein
Your displayer part should be of the {<part!} style (no start, with stop). There should be NO prepro sequence in your part. You should design your construction file to insert your part into plasmid pBca9495KC-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.
