Talk:Synthetic Biology:Vectors/Single copy plasmid: Difference between revisions
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*It seems like the most likely short-mid term problem is that a researcher would be uncertain as to which BioBrick vector they had, rather than the doomsday question of trying to work out if there is a BioBrick vector somewhere in the drink that turned Drew's hair [[Barcodes|pink]]. | *It seems like the most likely short-mid term problem is that a researcher would be uncertain as to which BioBrick vector they had, rather than the doomsday question of trying to work out if there is a BioBrick vector somewhere in the drink that turned Drew's hair [[Barcodes|pink]]. | ||
**Given this assumption, could we choose restriction sites, each of which are found uniquely in one of our BioBrick vectors? A researcher could just prep, digest and run on a gel to tell which vector they had.--[[User:Bcanton|BC]] | **Given this assumption, could we choose restriction sites, each of which are found uniquely in one of our BioBrick vectors? A researcher could just prep, digest and run on a gel to tell which vector they had.--[[User:Bcanton|BC]] | ||
***It might be useful to be able to tell the plasmid (and resistance) by colony PCR rather than a prep. A PCR requires less starting material. -[[User:Jkm|Jkm]] |
Revision as of 21:34, 6 December 2005
I was wondering about the arrangement of the VF/VR sites and the terminators. If the terminators were outside the VF/VR sites we would get somewhat longer sequencing reads. The way they are designed currently I suppose there would be less possibility for non-specific transcription so there are arguments either way --BC
Is there a plan for the barcode?
- Should the barcode only be readable by sequencing or is it sufficient to just look for an amplified band in a PCR reaction.
- If PCR is sufficient we could build in a unique sequence just before the BB prefix and then design a reverse primer to that sequence to use along with VF.
- It seems like the most likely short-mid term problem is that a researcher would be uncertain as to which BioBrick vector they had, rather than the doomsday question of trying to work out if there is a BioBrick vector somewhere in the drink that turned Drew's hair pink.
- Given this assumption, could we choose restriction sites, each of which are found uniquely in one of our BioBrick vectors? A researcher could just prep, digest and run on a gel to tell which vector they had.--BC
- It might be useful to be able to tell the plasmid (and resistance) by colony PCR rather than a prep. A PCR requires less starting material. -Jkm
- Given this assumption, could we choose restriction sites, each of which are found uniquely in one of our BioBrick vectors? A researcher could just prep, digest and run on a gel to tell which vector they had.--BC