Talk:Real-time PCR: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| (3 intermediate revisions by one other user not shown) | |||
| Line 1: | Line 1: | ||
==The Babel effect== | ==The Babel effect== | ||
I originally thought to use Q-PCR as the title for a page describing QRT-PCR because: | |||
* it's short (compare: quantitative reverse transcriptase PCR = QRT-PCR or RT-RT-PCR) | * it's short (compare: quantitative reverse transcriptase PCR = QRT-PCR or RT-RT-PCR) | ||
* it indicates what this technique is about: quantification (would you know what the technique is for if you had never heard about it and just read the term "real time PCR"?) | * it indicates what this technique is about: quantification (would you know what the technique is for if you had never heard about it and just read the term "real time PCR"?) | ||
Q-PCR with previous reverse transcription could be put separately but since Q-PCR is usually preceeded by RT, I thought it best to keep things together here. | Q-PCR with previous reverse transcription could be put separately but since Q-PCR is usually preceeded by RT, I thought it best to keep things together here. | ||
[[User:Jasu|Jasu]] 13:47, 26 March 2007 (EDT) | [[User:Jasu|Jasu]] 13:47, 26 March 2007 (EDT), edited [[User:Jakob Suckale|Jakob Suckale]] 10:15, 14 January 2008 (CST) | ||
*'''[[User:Reshma P. Shetty|Reshma]] 15:37, 9 October 2007 (CDT)''': I think it might be worth distinguishing between the different variants of quantitative PCR. You're right that Q-PCR is normally preceded by reverse transcription and that normally when people talk about Q-PCR, they mean real time PCR. But neither of these has to be the case. So it might be worth making the Q-PCR page discuss all the variants so that people can understand what each variant does. | *'''[[User:Reshma P. Shetty|Reshma]] 15:37, 9 October 2007 (CDT)''': I think it might be worth distinguishing between the different variants of quantitative PCR. You're right that Q-PCR is normally preceded by reverse transcription and that normally when people talk about Q-PCR, they mean real time PCR. But neither of these has to be the case. So it might be worth making the Q-PCR page discuss all the variants so that people can understand what each variant does. | ||
:* Hi Reshma, you do have a point that quantitative PCR is not always ''real time reverse transcribed PCR'' and this is why we should have different pages. I do, however, think that most people looking for qPCR actually mean ''real time reverse transcribed/transcription PCR'', since endpoint PCR is not used much anymore especially for publication data. The same is probably true for real-time PCR which in most cases also means real-time PCR of cDNA/reverse transcribed RNA. I'll make sure to link these pages to [[PCR techniques]] as a central reference point to reduce the confusion created by these terms. [[User:Jakob Suckale|Jakob Suckale]] 10:15, 14 January 2008 (CST) | |||
*'''[[User:Reshma P. Shetty|Reshma]] 16:20, 9 October 2007 (CDT)''': I moved some pages around. See [[PCR techniques]] for the complete list. | |||
==Thanks== | ==Thanks== | ||
[[Image:Sunflower thanks.png]] Mille grazie a Mile. Vielen Dank an Sandra. Thanks for getting me started with Q-PCR. [[User:Jasu|Jasu]] 10:37, 26 March 2007 (EDT) | [[Image:Sunflower thanks.png]] Mille grazie a Mile. Vielen Dank an Sandra. Thanks for getting me started with Q-PCR. [[User:Jasu|Jasu]] 10:37, 26 March 2007 (EDT) | ||
Latest revision as of 09:15, 14 January 2008
The Babel effect
I originally thought to use Q-PCR as the title for a page describing QRT-PCR because:
- it's short (compare: quantitative reverse transcriptase PCR = QRT-PCR or RT-RT-PCR)
- it indicates what this technique is about: quantification (would you know what the technique is for if you had never heard about it and just read the term "real time PCR"?)
Q-PCR with previous reverse transcription could be put separately but since Q-PCR is usually preceeded by RT, I thought it best to keep things together here. Jasu 13:47, 26 March 2007 (EDT), edited Jakob Suckale 10:15, 14 January 2008 (CST)
- Reshma 15:37, 9 October 2007 (CDT): I think it might be worth distinguishing between the different variants of quantitative PCR. You're right that Q-PCR is normally preceded by reverse transcription and that normally when people talk about Q-PCR, they mean real time PCR. But neither of these has to be the case. So it might be worth making the Q-PCR page discuss all the variants so that people can understand what each variant does.
- Hi Reshma, you do have a point that quantitative PCR is not always real time reverse transcribed PCR and this is why we should have different pages. I do, however, think that most people looking for qPCR actually mean real time reverse transcribed/transcription PCR, since endpoint PCR is not used much anymore especially for publication data. The same is probably true for real-time PCR which in most cases also means real-time PCR of cDNA/reverse transcribed RNA. I'll make sure to link these pages to PCR techniques as a central reference point to reduce the confusion created by these terms. Jakob Suckale 10:15, 14 January 2008 (CST)
- Reshma 16:20, 9 October 2007 (CDT): I moved some pages around. See PCR techniques for the complete list.
Thanks
Mille grazie a Mile. Vielen Dank an Sandra. Thanks for getting me started with Q-PCR. Jasu 10:37, 26 March 2007 (EDT)
