Talk:20.109(S10):Induce protein and evaluate DNA (Day5)

Examples of induced (left) and control (right) cell pellets

TR O/N Cultures and Gels

Below you should list the OD₆₀₀ values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group	OD WT	OD M124S	OD Chosen mutant	Pellets yellow?
Red	0.229	0.302	0.251	All bright yellow-green (after O/N). [All pellets were slightly yellow. Pellet of wildtype had more green than the others. We left them to grow overnight]
Orange	0.227 (0.1713)	0.257 (0.1823)	0.263 (0.1624)	All bright yellow-green (after O/N). [Slightly green. Overnight at RT.]
Yellow	0.232 (.152)	0.244 (.151)	0.266(.146)	All bright yellow-green (after O/N). [All pellets were slightly yellow, but we decided to place them in the shaker at room temp. overnight.]
Green	0.225	0.235	0.226	All bright yellow-green (after O/N).
Blue	0.231 (0.169)	0.271 (0.205)	0.218	All bright yellow-green (after O/N). [Slightly yellow except for M124S sample]
Pink	0.215	0.266	0.210	All bright yellow-green (after O/N).
Purple	0.243 (0.144)	0.237 (0.148)	0.183 (0.105)	All bright yellow-green (after O/N). [Due to the imperfect (sadly) nature of our pellet, we left the samples at room temperature shaker overnight. Also, regarding our gel, we placed the 1kbp ladder in lane 8, not lane 3.]

Gels

T/R Red

T/R Orange

T/R Yellow

T/R Green

T/R Blue

T/R Pink

T/R Purple

WF O/N Cultures and Gels

Below you should list the OD₆₀₀ values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group	OD WT	OD M124S	OD Chosen mutant	Pellets yellow?
Red	0.206	0.283	0.206	All bright yellow-green (after O/N). [Yes, except M124S is faint yellow so we decided to go overnight]
Orange	0.214	0.290	0.208	All bright yellow-green (after O/N). [yes]
Yellow	0.249	0.237	E67K2; OD = 0.232	yes; they were yellow so we decided not to go overnight.
Green	0.238	0.273	0.214	All bright yellow-green (after O/N). [NO]
Blue	.281	.296	.242	Yes
Pink	0.246	0.262	0.191	The pellet in WT and E84G were bright yellow after 2 hours and 21 min. M124S was white-ish but was yellow after incubating an extra 15 minutes.
Purple	.176	.192	.109	Yes

Gels

W/F Red

W/F Orange

W/F Yellow

W/F Green

W/F Blue

W/F Pink

W/F Purple