T broth

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T-Broth (not to be confused with Terrific broth)


  1. 10 g Bacto-tryptone
  2. 5 g NaCl


  1. Mix dry ingredients and add distilled water up to 1 Liter
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes

Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen.


Studier, FW Virology 39:562 (1969) Link: Pubmed

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